| Day 1 |
|
|
| 9-10:30h |
Second and third generation sequencing technologies |
|
| 10:30-12h |
Introduction to RNAseq pipeline and Quality controls |
|
| 13-17h |
Practical session Linux and R |
|
| Day 2 |
|
|
| 9-10:30h |
Alignment, mapping and read counts |
|
| 10:30-12h |
Practical session Alignment I |
|
| 13-17h |
Practical session Alignment II |
|
| Day 3 |
|
|
| 9-10:30h |
Differential gene expression |
|
| 10:30-12h |
Practical session Differential gene expression |
|
| 13-17h |
Practical session Differential gene expression II |
|
| Day 4 |
|
|
| 9-10:30h |
Transcriptome Assembly and IsoSeq |
|
| 10:30-12h |
Practical session Isoseq |
|
| 13-17h |
Practical session de novo assembly (illumina) |
|
| Day 5 |
|
|
| 9-10:30h |
Functional analysis and introduction to gene annotation |
| 10:30-12h |
Single-cell gene expression |
|
| 13-15h |
Practical session Functional analysis |
|
| 15-17hs |
Wrap up |
|
