LymphoRioMove 2025
International Course on Immunotherapies and Lab Tools in the Era of Precision Medicine
Fiocruz, Rio de Janeiro  –  September, 03-05
 

* August 28th–September 2nd  – Theoretical Activities with previous reading (material on the AVA/Campus Virtual, and/or sent by e-mail)

September 03 (Wednesday)
Morning (Theoretical Activities):
09:00 – Introduction to the course dynamics

09:30 – 11:00 – Lectures:
Actinopathies and WAS – uncovering how patient mutations modulate WASP activity –  Lisa Westerberg (Karolinska Institutet, Stockholm)
 
RENOMIEII – The Brazilian Genomic Network on Inborn Errors of Immunity: Progress and Challenges  –  Zilton Vasconcelos (Fernandes Figueira Institute/Fiocruz)
11:00 - 12:00 – Presentation of the groups of students: sub-course theme, the mini-project to be elaborated and the proposed practice
* Auditorium Maria Deane, Pav. Leonidas Deane, 1st floor (campus Manguinhos)
 
Afternoon (Lab Practices): 13:30 - 18:30
Sub-course 1: Cell electroporation, Ficoll demonstration, and purification
* Lab. de Pesquisas sobre o Timo (LPT), Pav. Leonidas Deane, sala 506 (campus Manguinhos)

Sub-course 2: Cell transfection and sorting of GFPlow and GFPhigh cells.
* Lab. de Pesquisas sobre o Timo (LPT), Pav. Leonidas Deane, sala 506 (campus Manguinhos)

Sub-course 3 and Sub-course 4: Dealing with specific cases of Inborn Errors of Immunity (IEI): case presentation, exploration through genomic and computational modelling tools
* Módulo de expansão do Ensino, sala 13,  Pav. Arthur Neiva (campus Manguinhos)


September 04 (Thursday):
Morning (Lab Practices): 09:00 - 12:00
Sub-course 1: Cell labeling and flow cytometry acquisition
* LPT, Pav. Leonidas Deane, sala 506 (campus Manguinhos)

Sub-course 2: Hands-on Ab modelling and structural analysis
* Módulo de expansão do Ensino, sala 13,  Pav. Arthur Neiva (campus Manguinhos)

Sub-course 3: Lab tools for exploring inflammation biomarkers: IEI sample preparation
* Lab. de Tecnologia Imunológica (LATIM), Pavilhão Rocha Lima, sala 414 (campus Manguinhos)

Sub-course 4: Plate preparation
Lecture:  Exploring the HCS (High-Content Screening) –  Lucas Rizzo (Thermo Fisher Scientific, São Paulo)
* LPT, Pav. Leonidas Deane, sala 506 (campus Manguinhos)
 
Afternoon (Lab Practices): 13:30 - 18:30
Sub-course 1: Flow cytometry analysis
* LPT, Pav. Leonidas Deane, sala 506 (campus Manguinhos)

Sub-course 2: Antibody expression analysis (flow cytometry, ELISA)
* LPT, Pav. Leonidas Deane, sala 506 (campus Manguinhos)

Sub-course 3: Multiplex analysis
* Lab. de Tecnologia Imunológica (LATIM), Pavilhão Rocha Lima, sala 414 (campus Manguinhos)

Sub-course 4: Plate acquisition and imaging analysis
* Lab. de Análise Imunomolecular (LANIM), Bio-Manguinhos (campus Manguinhos)
 
All groups: Preparation for the Presentation of the results

September 05 (Friday):
Morning:
08:30 - 10:00 - Preparation for the Presentation of the results
10:00 - 12:00: Lectures
Immune Checkpoint Signaling: Mechanism and Regulation – Renaud Lesourne (INFINITy/INSERM, Toulouse)
AI and Precision Medicine – Helder Nakaya (Einstein São Paulo, USP)
* Auditorium Emmanuel Dias, Pav. Arthur Neiva (campus Manguinhos)
 
Afternoon: Auditorium Maria Deane Dias, Pav. Leonidas Deane (campus Manguinhos)
13:30 - 16:30: Presentation of the results by the groups and discussion
16:30 – 17:00: Course closing